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rf-cloning troubles

Topic	Replies	Views	Last post
31 RF cloning secondary PCR troubles. by Abhinit	1	2,613	2017-12-18 15:20:36 by NagarA
32 Problem with Secondary PCR by gunception	7	14,098	2017-12-02 15:45:32 by Steve Bond
33 DpnI: Heat Inactivation Necessary or Detrimental? by ChrisC	1	7,714	2017-08-29 12:38:13 by Steve Bond
34 only original (un-edited) plasmids grow by snissim	1	4,834	2017-07-18 22:04:34 by Steve Bond
35 Secondary PCR not working by asilbering	5	10,201	2017-02-28 14:45:56 by asilbering
36 Rf Cloning false positives by filipebaeta	1	4,589	2017-01-23 15:49:44 by Steve Bond
37 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	5,039	2016-10-05 10:55:01 by Steve Bond
38 Relevance of DpnI treatment? by Alexander	4	19,463	2016-05-09 12:37:42 by CharlesPt
39 Amount of mega primer/plasmids second PCR. by Jan	5	13,117	2016-05-09 12:36:35 by CharlesPt
40 Problem with RF cloning by shrikant	1	5,120	2016-05-01 14:06:31 by Steve Bond
41 No colonies & no band on agarose gel after 2°PCR by afahrny	3	9,184	2015-12-21 11:24:24 by Steve Bond
42 to design an RF cloning project with the website by mandyscott	0	7,774	2015-12-21 06:00:15 by mandyscott
43 Secondary PCR does not work by tgattegno	8	18,264	2015-09-14 12:38:58 by Steve Bond
44 No band for products at all after primary PCR by bubunmicro@gmail.com	7	16,937	2015-06-30 20:14:40 by Steve Bond
45 RF-cloning to do mutagenesis? by erin.green	6	12,788	2015-02-18 17:16:07 by Steve Bond
46 Procedure after DpnI digestion by hongrulin	4	12,443	2015-02-18 17:15:33 by Steve Bond
47 DpnI by Sand	1	5,757	2015-01-30 16:15:24 by Steve Bond
48 Is phosphorilation needed? by Dungeonlord	18	41,839	2015-01-24 00:10:26 by Steve Bond
49 2nd PCR not working by ZacJ	8	69,723	2014-12-16 16:58:59 by ayidazagu
50 RFP + gene of interest RF cloning into vector in place of GFP ? by vsustar	2	20,973	2014-12-14 23:22:26 by LucilleHib
51 Problematic to say the least by pcr	7	19,731	2014-12-10 22:51:54 by Steve Bond
52 Insert is a very small tag - confused about the protocol? by Cate	1	7,463	2014-12-10 16:42:43 by Steve Bond
53 More than 3 fragments by dcunha	1	4,951	2014-12-08 21:44:34 by Steve Bond
54 empty vector by vithya	1	5,645	2014-10-23 18:45:23 by Steve Bond
55 Question on the basics by Sand	3	9,238	2014-08-11 20:08:05 by Steve Bond
56 Cloningtroubles when 'swapping' genes by Meyana	6	14,396	2014-08-05 11:02:03 by Steve Bond
57 Removing part by Sand	3	8,079	2014-07-26 15:03:44 by Steve Bond
58 Length of plasmid by Jan	5	14,201	2014-07-25 13:54:12 by Steve Bond
59 Cloning not working! Any advice? by erin.green	5	13,200	2014-07-13 15:30:18 by Steve Bond
60 Always "empty" vectors...? by Tiago	4	19,948	2014-07-10 10:25:05 by Tiago

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