Anything and everything cloning: Go...
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Dear all,
I am a complete newbie in this technique and I was wondering if it is possible to (at the same time as inserting a new piece) remove a piece from your vector.
My goal is actually to replace a part of my plasmid with another part.
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You sure can!
When you use the website to design your project, place the '!' points on either side of the sequence you want removed.
If you want, I can have a look at your project before you order the primers.
-Steve
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You sure can!
When you use the website to design your project, place the '!' points on either side of the sequence you want removed.
If you want, I can have a look at your project before you order the primers.
-Steve
If I understand you correctly, than the piece of the plasmid between the "!" marks will be removed and replaced by the insert , right?
Or?
eg.: ...TTTTTT ! AAAAAA ! TTTTTTT...
and the insert would be GGGG
than the plasmid would become ....TTTTT GGGG TTTTTT... ?
It would be nice yes, if you take a look at it. I will get back in touch with the sequence of the plasmid and the gene I would like to introduce.
Simple put: I have a plasmid in which I want to remove a part and "replace" it with another part.
The plasmid however is rather big!
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Yep, you've got it. Play around with the tool a little, and the results should be pretty intuitive.
-Steve
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