RF-Cloning.org Forum

dcunha

Member

Registered: 2014-12-08

Posts: 1

More than 3 fragments

Is it possible to insert 4 fragments in a vector using the RF-CLONING? If Yes, which protocol should I use?

Steve Bond

Administrator

Registered: 2014-01-23

Posts: 130

Re: More than 3 fragments

Hmm, might be a bit difficult. Check out the following method by Peleg and Unger: doi 10.1007/978-1-62703-764-8_6
(PM me if you run into the paywall, I'll send the pdf)
I haven't tried it myself, so I can't really advise you much more than this. Please let us know though if you have any success (or failure).
-Steve