RF-Cloning.org Forum

ksrmaataa

Member

Registered: 2022-02-14

Posts: 1

how to utilize rf cloning protocol for deleting DNA

Can you recommend how to modify rf cloning protocol so that it would allow me to create a plasmid with a 1 kb internal deletion?  Do you think it would work for deleting 1kb fragment?

Steve Bond

Administrator

Registered: 2014-01-23

Posts: 130

Re: how to utilize rf cloning protocol for deleting DNA

Hey there,
Yes, a deletion is possible. Specify one additional base in the deleted sequence (either with exclamation points in the sequence or by specify the positions in the text fields), then include that single base in the insertion field. This will give you the deletion you desire.
-Steve