RF-Cloning.org Forum

kjkjindal

Member

Registered: 2020-07-14

Posts: 1

Using electrocompetent cells

Hi, I am looking to use RF cloning to clone a complex library of barcodes. Has anyone tried using it with electro-competent cells? Do the PCR products need to be purified prior to use in electroporation?

Thanks

Steve Bond

Administrator

Registered: 2014-01-23

Posts: 130

Re: Using electrocompetent cells

You should probably treat the input DNA the same as you would following traditional ligation; if you need high efficiency, the salts in the sample will interfere with electroporation. I haven't tried electroporation in this context personally but don't see any obvious reason not to.
My only concern is that many projects see a high number of negative clones, so you may not get a great library. Seems like a 'try it and see' sort of situation.
Best of luck,
-Steve