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rf-cloning troubles

Topic	Replies	Views	Last post
2,731 Amount of mega primer/plasmids second PCR. by Jan	5	17,009	2016-05-09 12:36:35 by CharlesPt
2,732 Problem with RF cloning by shrikant	1	7,253	2016-05-01 14:06:31 by Steve Bond
2,733 No colonies & no band on agarose gel after 2°PCR by afahrny	3	13,016	2015-12-21 11:24:24 by Steve Bond
2,734 to design an RF cloning project with the website by mandyscott	0	9,254	2015-12-21 06:00:15 by mandyscott
2,735 Secondary PCR does not work by tgattegno	8	24,571	2015-09-14 12:38:58 by Steve Bond
2,736 No band for products at all after primary PCR by bubunmicro@gmail.com	7	27,967	2015-06-30 20:14:40 by Steve Bond
2,737 suggestions for primary PCR by vithya	5	17,908	2015-05-10 14:22:04 by Steve Bond
2,738 RF-cloning to do mutagenesis? by erin.green	6	18,102	2015-02-18 17:16:07 by Steve Bond
2,739 Procedure after DpnI digestion by hongrulin	4	16,935	2015-02-18 17:15:33 by Steve Bond
2,740 DpnI by Sand	1	7,864	2015-01-30 16:15:24 by Steve Bond
2,741 Is phosphorilation needed? by Dungeonlord	18	55,251	2015-01-24 00:10:26 by Steve Bond
2,742 2nd PCR not working by ZacJ	8	80,329	2014-12-16 16:58:59 by ayidazagu
2,743 RFP + gene of interest RF cloning into vector in place of GFP ? by vsustar	2	31,005	2014-12-14 23:22:26 by LucilleHib
2,744 Problematic to say the least by pcr	7	25,761	2014-12-10 22:51:54 by Steve Bond
2,745 Insert is a very small tag - confused about the protocol? by Cate	1	9,515	2014-12-10 16:42:43 by Steve Bond
2,746 More than 3 fragments by dcunha	1	7,062	2014-12-08 21:44:34 by Steve Bond
2,747 empty vector by vithya	1	7,564	2014-10-23 18:45:23 by Steve Bond
2,748 Question on the basics by Sand	3	13,077	2014-08-11 20:08:05 by Steve Bond
2,749 Cloningtroubles when 'swapping' genes by Meyana	6	19,710	2014-08-05 11:02:03 by Steve Bond
2,750 Removing part by Sand	3	11,620	2014-07-26 15:03:44 by Steve Bond
2,751 Length of plasmid by Jan	5	18,759	2014-07-25 13:54:12 by Steve Bond
2,752 Cloning not working! Any advice? by erin.green	5	18,661	2014-07-13 15:30:18 by Steve Bond
2,753 Always "empty" vectors...? by Tiago	4	24,028	2014-07-10 10:25:05 by Tiago
2,754 Suggestion for Primer design and conditions by Tamjeed	7	18,298	2014-06-30 19:55:21 by Danielbut
2,755 Any suggestions? by galcafri	9	25,465	2014-06-04 22:52:45 by Steve Bond
2,756 any ideas on what I am doing wrong? by ebeisn2	4	17,820	2014-02-07 22:59:45 by Steve Bond

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