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rf-cloning troubles

Topic	Replies	Views	Last post
2,671 Cant get any colonies by asli84	1	4,390	2020-03-03 02:06:04 by Steve Bond
2,672 Issues with the Secondary PCR reaction by koikkarah93	1	5,003	2019-08-27 00:16:59 by Steve Bond
2,673 trouble with 2nd PCR! Please help by hyla	4	9,170	2019-06-23 12:41:59 by Steve Bond
2,674 Colony PCR for small inserts by Ranjani	3	9,149	2018-08-11 14:32:29 by Steve Bond
2,675 Fake colony by jailanian16	11	23,191	2018-05-31 15:21:04 by Steve Bond
2,676 Any data analyzing tool suggestion? by bioscience	2	7,749	2018-04-13 16:47:03 by Steve Bond
2,677 2nd PCR not working by ellenmcmullen	1	6,335	2018-03-11 13:13:42 by Steve Bond
2,678 Secondary PCR trouble by abhinit	3	9,740	2017-12-19 15:30:38 by abhinit
2,679 RF cloning secondary PCR troubles. by Abhinit	1	4,143	2017-12-18 15:20:36 by NagarA
2,680 Problem with Secondary PCR by gunception	7	21,770	2017-12-02 15:45:32 by Steve Bond
2,681 only original (un-edited) plasmids grow by snissim	1	7,350	2017-07-18 22:04:34 by Steve Bond
2,682 Insert size by cblank	3	11,819	2017-06-23 12:59:45 by Steve Bond
2,683 Secondary PCR not working by asilbering	5	15,608	2017-02-28 14:45:56 by asilbering
2,684 Rf Cloning false positives by filipebaeta	1	7,058	2017-01-23 15:49:44 by Steve Bond
2,685 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	7,499	2016-10-05 10:55:01 by Steve Bond
2,686 Relevance of DpnI treatment? by Alexander	4	23,744	2016-05-09 12:37:42 by CharlesPt
2,687 Amount of mega primer/plasmids second PCR. by Jan	5	17,447	2016-05-09 12:36:35 by CharlesPt
2,688 Problem with RF cloning by shrikant	1	7,537	2016-05-01 14:06:31 by Steve Bond
2,689 No colonies & no band on agarose gel after 2°PCR by afahrny	3	13,453	2015-12-21 11:24:24 by Steve Bond
2,690 to design an RF cloning project with the website by mandyscott	0	9,441	2015-12-21 06:00:15 by mandyscott
2,691 No band for products at all after primary PCR by bubunmicro@gmail.com	7	28,579	2015-06-30 20:14:40 by Steve Bond
2,692 suggestions for primary PCR by vithya	5	18,421	2015-05-10 14:22:04 by Steve Bond
2,693 RF-cloning to do mutagenesis? by erin.green	6	18,639	2015-02-18 17:16:07 by Steve Bond
2,694 Procedure after DpnI digestion by hongrulin	4	17,452	2015-02-18 17:15:33 by Steve Bond
2,695 DpnI by Sand	1	8,086	2015-01-30 16:15:24 by Steve Bond
2,696 Is phosphorilation needed? by Dungeonlord	18	56,841	2015-01-24 00:10:26 by Steve Bond
2,697 2nd PCR not working by ZacJ	8	81,091	2014-12-16 16:58:59 by ayidazagu
2,698 RFP + gene of interest RF cloning into vector in place of GFP ? by vsustar	2	31,317	2014-12-14 23:22:26 by LucilleHib
2,699 Problematic to say the least by pcr	7	26,397	2014-12-10 22:51:54 by Steve Bond
2,700 Insert is a very small tag - confused about the protocol? by Cate	1	9,740	2014-12-10 16:42:43 by Steve Bond

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