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rf-cloning troubles

Topic	Replies	Views	Last post
2,461 Any data analyzing tool suggestion? by bioscience	2	7,722	2018-04-13 16:47:03 by Steve Bond
2,462 2nd PCR not working by ellenmcmullen	1	6,318	2018-03-11 13:13:42 by Steve Bond
2,463 Secondary PCR trouble by abhinit	3	9,703	2017-12-19 15:30:38 by abhinit
2,464 RF cloning secondary PCR troubles. by Abhinit	1	4,129	2017-12-18 15:20:36 by NagarA
2,465 Problem with Secondary PCR by gunception	7	21,715	2017-12-02 15:45:32 by Steve Bond
2,466 only original (un-edited) plasmids grow by snissim	1	7,331	2017-07-18 22:04:34 by Steve Bond
2,467 Insert size by cblank	3	11,767	2017-06-23 12:59:45 by Steve Bond
2,468 Secondary PCR not working by asilbering	5	15,558	2017-02-28 14:45:56 by asilbering
2,469 Rf Cloning false positives by filipebaeta	1	7,036	2017-01-23 15:49:44 by Steve Bond
2,470 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	7,476	2016-10-05 10:55:01 by Steve Bond
2,471 Relevance of DpnI treatment? by Alexander	4	23,705	2016-05-09 12:37:42 by CharlesPt
2,472 Amount of mega primer/plasmids second PCR. by Jan	5	17,405	2016-05-09 12:36:35 by CharlesPt
2,473 Problem with RF cloning by shrikant	1	7,512	2016-05-01 14:06:31 by Steve Bond
2,474 No colonies & no band on agarose gel after 2°PCR by afahrny	3	13,421	2015-12-21 11:24:24 by Steve Bond
2,475 to design an RF cloning project with the website by mandyscott	0	9,429	2015-12-21 06:00:15 by mandyscott
2,476 No band for products at all after primary PCR by bubunmicro@gmail.com	7	28,520	2015-06-30 20:14:40 by Steve Bond
2,477 suggestions for primary PCR by vithya	5	18,352	2015-05-10 14:22:04 by Steve Bond
2,478 RF-cloning to do mutagenesis? by erin.green	6	18,593	2015-02-18 17:16:07 by Steve Bond
2,479 Procedure after DpnI digestion by hongrulin	4	17,395	2015-02-18 17:15:33 by Steve Bond
2,480 DpnI by Sand	1	8,066	2015-01-30 16:15:24 by Steve Bond
2,481 Is phosphorilation needed? by Dungeonlord	18	56,670	2015-01-24 00:10:26 by Steve Bond
2,482 2nd PCR not working by ZacJ	8	81,023	2014-12-16 16:58:59 by ayidazagu
2,483 RFP + gene of interest RF cloning into vector in place of GFP ? by vsustar	2	31,289	2014-12-14 23:22:26 by LucilleHib
2,484 Problematic to say the least by pcr	7	26,346	2014-12-10 22:51:54 by Steve Bond
2,485 Insert is a very small tag - confused about the protocol? by Cate	1	9,716	2014-12-10 16:42:43 by Steve Bond
2,486 More than 3 fragments by dcunha	1	7,254	2014-12-08 21:44:34 by Steve Bond
2,487 empty vector by vithya	1	7,779	2014-10-23 18:45:23 by Steve Bond
2,488 Question on the basics by Sand	3	13,403	2014-08-11 20:08:05 by Steve Bond
2,489 Cloningtroubles when 'swapping' genes by Meyana	6	20,293	2014-08-05 11:02:03 by Steve Bond
2,490 Removing part by Sand	3	11,944	2014-07-26 15:03:44 by Steve Bond

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