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rf-cloning troubles

Topic	Replies	Views	Last post
2,071 Unspecific binding of primers to target by rfuser	1	3,683	2021-03-06 16:23:22 by Steve Bond
2,072 Secondary PCR transformation not working by janhavi23	4	5,175	2021-02-14 16:51:47 by Steve Bond
2,073 No positive colony after 2nd PCR,is high T-A concentration a problems? by linh_	2	4,180	2021-01-18 00:36:19 by linh_
2,074 Second PCR not working by Manu	4	5,729	2021-01-15 07:29:32 by linh_
2,075 Ordered primers that make the entire insert - overlap on both ends by jk1234	1	3,177	2020-08-30 09:08:34 by Steve Bond
2,076 Using electrocompetent cells by kjkjindal	1	3,925	2020-07-14 09:11:06 by Steve Bond
2,077 Cant get any colonies by asli84	1	4,086	2020-03-03 02:06:04 by Steve Bond
2,078 Issues with the Secondary PCR reaction by koikkarah93	1	4,701	2019-08-27 00:16:59 by Steve Bond
2,079 trouble with 2nd PCR! Please help by hyla	4	8,615	2019-06-23 12:41:59 by Steve Bond
2,080 Colony PCR for small inserts by Ranjani	3	8,654	2018-08-11 14:32:29 by Steve Bond
2,081 Fake colony by jailanian16	11	21,862	2018-05-31 15:21:04 by Steve Bond
2,082 Any data analyzing tool suggestion? by bioscience	2	7,434	2018-04-13 16:47:03 by Steve Bond
2,083 2nd PCR not working by ellenmcmullen	1	6,070	2018-03-11 13:13:42 by Steve Bond
2,084 Secondary PCR trouble by abhinit	3	9,236	2017-12-19 15:30:38 by abhinit
2,085 RF cloning secondary PCR troubles. by Abhinit	1	3,940	2017-12-18 15:20:36 by NagarA
2,086 Problem with Secondary PCR by gunception	7	20,865	2017-12-02 15:45:32 by Steve Bond
2,087 DpnI: Heat Inactivation Necessary or Detrimental? by ChrisC	1	11,912	2017-08-29 12:38:13 by Steve Bond
2,088 only original (un-edited) plasmids grow by snissim	1	7,044	2017-07-18 22:04:34 by Steve Bond
2,089 Insert size by cblank	3	11,309	2017-06-23 12:59:45 by Steve Bond
2,090 Secondary PCR not working by asilbering	5	14,963	2017-02-28 14:45:56 by asilbering
2,091 Rf Cloning false positives by filipebaeta	1	6,740	2017-01-23 15:49:44 by Steve Bond
2,092 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	7,202	2016-10-05 10:55:01 by Steve Bond
2,093 Relevance of DpnI treatment? by Alexander	4	23,199	2016-05-09 12:37:42 by CharlesPt
2,094 Amount of mega primer/plasmids second PCR. by Jan	5	16,870	2016-05-09 12:36:35 by CharlesPt
2,095 Problem with RF cloning by shrikant	1	7,174	2016-05-01 14:06:31 by Steve Bond
2,096 No colonies & no band on agarose gel after 2°PCR by afahrny	3	12,887	2015-12-21 11:24:24 by Steve Bond
2,097 to design an RF cloning project with the website by mandyscott	0	9,211	2015-12-21 06:00:15 by mandyscott
2,098 Secondary PCR does not work by tgattegno	8	24,334	2015-09-14 12:38:58 by Steve Bond
2,099 No band for products at all after primary PCR by bubunmicro@gmail.com	7	27,772	2015-06-30 20:14:40 by Steve Bond
2,100 suggestions for primary PCR by vithya	5	17,744	2015-05-10 14:22:04 by Steve Bond

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