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rf-cloning troubles

Topic	Replies	Views	Last post
1,081 Relevance of DpnI treatment? by Alexander	4	23,554	2016-05-09 12:37:42 by CharlesPt
1,082 Amount of mega primer/plasmids second PCR. by Jan	5	17,223	2016-05-09 12:36:35 by CharlesPt
1,083 Problem with RF cloning by shrikant	1	7,398	2016-05-01 14:06:31 by Steve Bond
1,084 No colonies & no band on agarose gel after 2°PCR by afahrny	3	13,232	2015-12-21 11:24:24 by Steve Bond
1,085 to design an RF cloning project with the website by mandyscott	0	9,360	2015-12-21 06:00:15 by mandyscott
1,086 Secondary PCR does not work by tgattegno	8	24,904	2015-09-14 12:38:58 by Steve Bond
1,087 No band for products at all after primary PCR by bubunmicro@gmail.com	7	28,285	2015-06-30 20:14:40 by Steve Bond
1,088 suggestions for primary PCR by vithya	5	18,161	2015-05-10 14:22:04 by Steve Bond
1,089 RF-cloning to do mutagenesis? by erin.green	6	18,377	2015-02-18 17:16:07 by Steve Bond
1,090 Procedure after DpnI digestion by hongrulin	4	17,173	2015-02-18 17:15:33 by Steve Bond
1,091 DpnI by Sand	1	7,982	2015-01-30 16:15:24 by Steve Bond
1,092 Is phosphorilation needed? by Dungeonlord	18	56,003	2015-01-24 00:10:26 by Steve Bond
1,093 2nd PCR not working by ZacJ	8	80,710	2014-12-16 16:58:59 by ayidazagu
1,094 RFP + gene of interest RF cloning into vector in place of GFP ? by vsustar	2	31,165	2014-12-14 23:22:26 by LucilleHib
1,095 Problematic to say the least by pcr	7	26,102	2014-12-10 22:51:54 by Steve Bond
1,096 Insert is a very small tag - confused about the protocol? by Cate	1	9,641	2014-12-10 16:42:43 by Steve Bond
1,097 More than 3 fragments by dcunha	1	7,172	2014-12-08 21:44:34 by Steve Bond
1,098 empty vector by vithya	1	7,688	2014-10-23 18:45:23 by Steve Bond
1,099 Question on the basics by Sand	3	13,272	2014-08-11 20:08:05 by Steve Bond
1,100 Cloningtroubles when 'swapping' genes by Meyana	6	20,029	2014-08-05 11:02:03 by Steve Bond
1,101 Removing part by Sand	3	11,800	2014-07-26 15:03:44 by Steve Bond
1,102 Length of plasmid by Jan	5	19,025	2014-07-25 13:54:12 by Steve Bond
1,103 Cloning not working! Any advice? by erin.green	5	19,025	2014-07-13 15:30:18 by Steve Bond
1,104 Always "empty" vectors...? by Tiago	4	24,329	2014-07-10 10:25:05 by Tiago
1,105 Suggestion for Primer design and conditions by Tamjeed	7	18,618	2014-06-30 19:55:21 by Danielbut
1,106 Any suggestions? by galcafri	9	25,846	2014-06-04 22:52:45 by Steve Bond
1,107 any ideas on what I am doing wrong? by ebeisn2	4	18,027	2014-02-07 22:59:45 by Steve Bond

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