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rf-cloning troubles

Topic	Replies	Views	Last post
991 Colony PCR for small inserts by Ranjani	3	8,951	2018-08-11 14:32:29 by Steve Bond
992 Fake colony by jailanian16	11	22,687	2018-05-31 15:21:04 by Steve Bond
993 Any data analyzing tool suggestion? by bioscience	2	7,608	2018-04-13 16:47:03 by Steve Bond
994 2nd PCR not working by ellenmcmullen	1	6,239	2018-03-11 13:13:42 by Steve Bond
995 Secondary PCR trouble by abhinit	3	9,557	2017-12-19 15:30:38 by abhinit
996 RF cloning secondary PCR troubles. by Abhinit	1	4,056	2017-12-18 15:20:36 by NagarA
997 Problem with Secondary PCR by gunception	7	21,421	2017-12-02 15:45:32 by Steve Bond
998 DpnI: Heat Inactivation Necessary or Detrimental? by ChrisC	1	12,205	2017-08-29 12:38:13 by Steve Bond
999 only original (un-edited) plasmids grow by snissim	1	7,236	2017-07-18 22:04:34 by Steve Bond
1,000 Insert size by cblank	3	11,625	2017-06-23 12:59:45 by Steve Bond
1,001 Secondary PCR not working by asilbering	5	15,369	2017-02-28 14:45:56 by asilbering
1,002 Rf Cloning false positives by filipebaeta	1	6,940	2017-01-23 15:49:44 by Steve Bond
1,003 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	7,380	2016-10-05 10:55:01 by Steve Bond
1,004 Relevance of DpnI treatment? by Alexander	4	23,540	2016-05-09 12:37:42 by CharlesPt
1,005 Amount of mega primer/plasmids second PCR. by Jan	5	17,216	2016-05-09 12:36:35 by CharlesPt
1,006 Problem with RF cloning by shrikant	1	7,385	2016-05-01 14:06:31 by Steve Bond
1,007 No colonies & no band on agarose gel after 2°PCR by afahrny	3	13,226	2015-12-21 11:24:24 by Steve Bond
1,008 to design an RF cloning project with the website by mandyscott	0	9,358	2015-12-21 06:00:15 by mandyscott
1,009 Secondary PCR does not work by tgattegno	8	24,896	2015-09-14 12:38:58 by Steve Bond
1,010 No band for products at all after primary PCR by bubunmicro@gmail.com	7	28,262	2015-06-30 20:14:40 by Steve Bond
1,011 suggestions for primary PCR by vithya	5	18,145	2015-05-10 14:22:04 by Steve Bond
1,012 RF-cloning to do mutagenesis? by erin.green	6	18,365	2015-02-18 17:16:07 by Steve Bond
1,013 Procedure after DpnI digestion by hongrulin	4	17,161	2015-02-18 17:15:33 by Steve Bond
1,014 DpnI by Sand	1	7,972	2015-01-30 16:15:24 by Steve Bond
1,015 Is phosphorilation needed? by Dungeonlord	18	55,969	2015-01-24 00:10:26 by Steve Bond
1,016 2nd PCR not working by ZacJ	8	80,688	2014-12-16 16:58:59 by ayidazagu
1,017 RFP + gene of interest RF cloning into vector in place of GFP ? by vsustar	2	31,155	2014-12-14 23:22:26 by LucilleHib
1,018 Problematic to say the least by pcr	7	26,094	2014-12-10 22:51:54 by Steve Bond
1,019 Insert is a very small tag - confused about the protocol? by Cate	1	9,633	2014-12-10 16:42:43 by Steve Bond
1,020 More than 3 fragments by dcunha	1	7,162	2014-12-08 21:44:34 by Steve Bond

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