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rf-cloning troubles

Topic	Replies	Views	Last post
4,411 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	7,657	2016-10-05 10:55:01 by Steve Bond
4,412 Relevance of DpnI treatment? by Alexander	4	24,072	2016-05-09 12:37:42 by CharlesPt
4,413 Amount of mega primer/plasmids second PCR. by Jan	5	17,833	2016-05-09 12:36:35 by CharlesPt
4,414 Problem with RF cloning by shrikant	1	7,729	2016-05-01 14:06:31 by Steve Bond
4,415 No colonies & no band on agarose gel after 2°PCR by afahrny	3	13,762	2015-12-21 11:24:24 by Steve Bond
4,416 to design an RF cloning project with the website by mandyscott	0	9,573	2015-12-21 06:00:15 by mandyscott
4,417 No band for products at all after primary PCR by bubunmicro@gmail.com	7	29,137	2015-06-30 20:14:40 by Steve Bond
4,418 suggestions for primary PCR by vithya	5	18,780	2015-05-10 14:22:04 by Steve Bond
4,419 RF-cloning to do mutagenesis? by erin.green	6	19,002	2015-02-18 17:16:07 by Steve Bond
4,420 Procedure after DpnI digestion by hongrulin	4	17,754	2015-02-18 17:15:33 by Steve Bond
4,421 DpnI by Sand	1	8,245	2015-01-30 16:15:24 by Steve Bond
4,422 Is phosphorilation needed? by Dungeonlord	18	57,979	2015-01-24 00:10:26 by Steve Bond
4,423 2nd PCR not working by ZacJ	8	81,729	2014-12-16 16:58:59 by ayidazagu
4,424 RFP + gene of interest RF cloning into vector in place of GFP ? by vsustar	2	31,536	2014-12-14 23:22:26 by LucilleHib
4,425 Problematic to say the least by pcr	7	26,835	2014-12-10 22:51:54 by Steve Bond
4,426 Insert is a very small tag - confused about the protocol? by Cate	1	9,904	2014-12-10 16:42:43 by Steve Bond
4,427 More than 3 fragments by dcunha	1	7,431	2014-12-08 21:44:34 by Steve Bond
4,428 empty vector by vithya	1	7,974	2014-10-23 18:45:23 by Steve Bond
4,429 Question on the basics by Sand	3	13,693	2014-08-11 20:08:05 by Steve Bond
4,430 Cloningtroubles when 'swapping' genes by Meyana	6	20,731	2014-08-05 11:02:03 by Steve Bond
4,431 Removing part by Sand	3	12,201	2014-07-26 15:03:44 by Steve Bond
4,432 Length of plasmid by Jan	5	19,676	2014-07-25 13:54:12 by Steve Bond
4,433 Cloning not working! Any advice? by erin.green	5	19,851	2014-07-13 15:30:18 by Steve Bond
4,434 Always "empty" vectors...? by Tiago	4	25,004	2014-07-10 10:25:05 by Tiago
4,435 Suggestion for Primer design and conditions by Tamjeed	7	19,266	2014-06-30 19:55:21 by Danielbut
4,436 Any suggestions? by galcafri	9	26,749	2014-06-04 22:52:45 by Steve Bond
4,437 any ideas on what I am doing wrong? by ebeisn2	4	18,468	2014-02-07 22:59:45 by Steve Bond

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