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rf-cloning troubles

Topic	Replies	Views	Last post
3,781 How to get insert:vector ratio in the 2' PCR? by minyoungfan33	1	7,587	2016-10-05 10:55:01 by Steve Bond
3,782 Relevance of DpnI treatment? by Alexander	4	23,933	2016-05-09 12:37:42 by CharlesPt
3,783 Amount of mega primer/plasmids second PCR. by Jan	5	17,692	2016-05-09 12:36:35 by CharlesPt
3,784 Problem with RF cloning by shrikant	1	7,662	2016-05-01 14:06:31 by Steve Bond
3,785 No colonies & no band on agarose gel after 2°PCR by afahrny	3	13,626	2015-12-21 11:24:24 by Steve Bond
3,786 to design an RF cloning project with the website by mandyscott	0	9,523	2015-12-21 06:00:15 by mandyscott
3,787 No band for products at all after primary PCR by bubunmicro@gmail.com	7	28,891	2015-06-30 20:14:40 by Steve Bond
3,788 suggestions for primary PCR by vithya	5	18,632	2015-05-10 14:22:04 by Steve Bond
3,789 RF-cloning to do mutagenesis? by erin.green	6	18,851	2015-02-18 17:16:07 by Steve Bond
3,790 Procedure after DpnI digestion by hongrulin	4	17,620	2015-02-18 17:15:33 by Steve Bond
3,791 DpnI by Sand	1	8,179	2015-01-30 16:15:24 by Steve Bond
3,792 Is phosphorilation needed? by Dungeonlord	18	57,416	2015-01-24 00:10:26 by Steve Bond
3,793 2nd PCR not working by ZacJ	8	81,490	2014-12-16 16:58:59 by ayidazagu
3,794 RFP + gene of interest RF cloning into vector in place of GFP ? by vsustar	2	31,448	2014-12-14 23:22:26 by LucilleHib
3,795 Problematic to say the least by pcr	7	26,628	2014-12-10 22:51:54 by Steve Bond
3,796 Insert is a very small tag - confused about the protocol? by Cate	1	9,840	2014-12-10 16:42:43 by Steve Bond
3,797 More than 3 fragments by dcunha	1	7,376	2014-12-08 21:44:34 by Steve Bond
3,798 empty vector by vithya	1	7,906	2014-10-23 18:45:23 by Steve Bond
3,799 Question on the basics by Sand	3	13,593	2014-08-11 20:08:05 by Steve Bond
3,800 Cloningtroubles when 'swapping' genes by Meyana	6	20,567	2014-08-05 11:02:03 by Steve Bond
3,801 Removing part by Sand	3	12,103	2014-07-26 15:03:44 by Steve Bond
3,802 Length of plasmid by Jan	5	19,541	2014-07-25 13:54:12 by Steve Bond
3,803 Cloning not working! Any advice? by erin.green	5	19,634	2014-07-13 15:30:18 by Steve Bond
3,804 Always "empty" vectors...? by Tiago	4	24,811	2014-07-10 10:25:05 by Tiago
3,805 Suggestion for Primer design and conditions by Tamjeed	7	19,123	2014-06-30 19:55:21 by Danielbut
3,806 Any suggestions? by galcafri	9	26,520	2014-06-04 22:52:45 by Steve Bond
3,807 any ideas on what I am doing wrong? by ebeisn2	4	18,354	2014-02-07 22:59:45 by Steve Bond

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