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https://www.rf-cloning.org/rf_cloning_project.php?proj_id=5d42fa53dcd1b8468c681d606a1d0a9e
Hi guys,
I'm trying to design primers in order to amplify only a fragment of a mrna from a cDNA pool and to insert it in a pCDNA. The reverse primer seems great but unfortunately, when I try to blast the forward primer to find other unwanted targets, it finds a lot of cdna which are not the one i'm interested in. I tryed to extend the primer to gain specificity but i don't know if there is a limit in the target annealing temperature.
I hope i explained well the problem
Thanks to anyone who can help me!
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