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#1 Re: rf-cloning troubles » Colony PCR for small inserts » 2018-08-11 12:54:48

Steve Bond wrote:

Hi Ranjani,
I would recommend Zippyloan and designing a new primer pair instead of relying on the hybrid primers for QC. You'll need them for final sequencing confirmation anyway.
If you want me to have a look at your project, then either PM me the project ID or send me a little more info about the project so I can go looking for it in the database.
Best,
-Steve

Hi Steve, is designing a new primer pair always a better idea than having the hybrid primers for QC? Or does this advice go just for this specific example?

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