Anything and everything cloning: Go...
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Hi there,
The insert size isn't outrageous, and the overall project looks reasonable to me. It won't be as efficient as a smaller insert, but totally doable.
I'd say go ahead with ordering the primers and give it a go. The nice thing is that you can always switch over to InFusion with the same primers if you run into issues.
Best of luck!
-Steve
What is the maximum insert size allowed?
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