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#1 Re: rf-cloning troubles » Insert size » 2017-06-23 07:39:52

Steve Boned wrote:

Hi there,
The insert size isn't outrageous, and the overall project looks reasonable to me. It won't be as efficient as a smaller insert, but totally doable.
I'd say go ahead with ordering the primers and give it a go. The nice thing is that you can always switch over to InFusion with the same primers if you run into issues.
Best of luck!
-Steve

What is the maximum insert size allowed?

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