RF-Cloning.org Forum

Hi!

First off, thanks for maintaining such a useful website!

I have a question before I begin RF-cloning. I designed this project because I'm having issues subcloning the old fashioned way, http://www.rf-cloning.org/rf_cloning_project.php?proj_id=2f0a4e5c32429107ef22b15e19d984d1 but before I order the primers I was wondering if there is a limit to the size of insert that works for RF cloning? Would you recommend Gibson or In-Fusion instead (I haven't used either before)? I've had great success with RF-cloning for small inserts and deletions before, but nothing of this size.

Thanks!