Anything and everything cloning: Go...
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Everything worked well and sequence was correct. Thanks for the help Steve!
Thanks Steve,
Primers have been ordered already..had to wait through a snow storm to get them. First round successful (making megaprimer) starting the second round right now.
Thanks again,
EH
I saw this post and didn't want to start a new question. I have a gene that I cloned which ended up with two single mutations about 200-300 bp apart. i was planing on making a megaprimer (i guess it is actually a large quick change primer about 200bp) of the wild type (without the mutations) for this region of the gene and use it in the 2nd pcr. I feel this should work no problem... any thoughts?
Thanks-
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