RF-Cloning.org Forum

We published a paper this spring vector containing ccdB-toxin  to select for transformants instead of using DpnI for selection.
Lund, B. A., Leiros, H.-K. S., & Bjerga, G. E. (2014). A high-throughput, restriction-free cloning and screening strategy based on ccdB-gene replacement. Microbial Cell Factories, 13(1), 38. doi:10.1186/1475-2859-13-38